KMID : 0895820140240030071
|
|
Journal of Oriental Rehabilitation Medicine 2014 Volume.24 No. 3 p.71 ~ p.85
|
|
Anti-allergic Effects of Samhwangsasim-tang (S?nhuangxiex?nt?ng) on Ovalbumin-induced Allergic Model in Mice
|
|
Choi Chong-Hwan
Keum Seon-Oh Lee Se-Won Kim Il-Hyun Lee Ha-Il Song Yuug-Sun
|
|
Abstract
|
|
|
Objectives : In this study, we investigated the inhibitory effects of Samhwangsasim-tang (S.H) on the allergic response caused by ovalbumin(OVA) sensitization and challenge in BALB/c mice.
Methods : The experimental animals were divided into five groups; 1) normal as negative control, 2) OVA-sensitized mice, 3) OVA-sensitized mice treated with 200 mg/kg of S.H 200, 4) OVA-sensitized mice treated with 400 mg/kg of S.H 400, and 5) OVA-sensitized mice treated with 5 mg/kg of Dexamethasone (Dex). Antigen sensitization for allergic mouse model was performed with twice injection of OVA for 2 weeks. After secondary injection, S.H was administrated orally into mice every day for 13 days and the inhibitory effect of S.H on allergic responses was evaluated.
Results : Treatment of S.H into allergic mice reduced significantly ear edema and infiltration of immune cells in ear tissues induced with OVA challenge in a dose-dependent manner. S.H reduced significantly the serum levels of Total Immunoglobulin(Ig)G and IgE, and particularly inhibited the production of OVA-specific IgE, but not OVA-specific IgG. The serum level of proinflammatory cytokine TNF-¥á and Th2-associated cytokine IL-4 also were significantly decreased by S.H adminstration in a dose denpendent manner. S.H attenuated OVA-induced secretion of IFN-¥ã, but not IL-12 which is a cytokine inducing the development of Th1 cells. It also reduced significantly the secretion of IL-4, which is a cytokine inducing the development of Th2 cells, after splenocytes were stimulated with OVA. However the secretion of IL-5 and IL-13 was influenced weakly or a little.
Conclusions : These results indicate that S.H could reduce the allergic response through inhibition of antigen-specific IgE and Th2-inducing cytokines. It suggest that S.H may be available clinically for the treatment of allergic patients.
|
|
KEYWORD
|
|
Samhwangsasim-tang, S.H, Allergic disease, Ovalbumin, Immunoglobulin, proinflammatory cytokine, Interleukin-4
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|